Induction of the CD24 surface antigen in primary undifferentiated human adipose progenitor cells by the hedgehog signaling pathway
Additional information
Authors
Muoio F.,
Campanella S.,
Harder Y.,
Tallone T.
Type
Journal Article
Year
2021
Language
English
Abstract
In the murine model system of adipogenesis, the CD24 cell surface protein represents a valuable marker to label undifferentiated adipose progenitor cells. Indeed, when injected into the residual fat pads of lipodystrophic mice, these CD24 positive cells reconstitute a normal white adipose tissue (WAT) depot. Unluckily, similar studies in humans are rare and incomplete. This is because it is impossible to obtain large numbers of primary CD24 positive human adipose stem cells (hASCs). This study shows that primary hASCs start to express the glycosylphosphatidylinositol (GPI)-anchored CD24 protein when cultured with a chemically defined medium supplemented with molecules that activate the Hedgehog (Hh) signaling pathway. Therefore, this in vitro system may help understand the biology and role in adipogenesis of the CD24-positive hASCs. The induced cells’ phenotype was studied by flow cytometry, Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) techniques, and their secretion profile. The results show that CD24 positive cells are early undifferentiated progenitors expressing molecules related to the angiogenic pathway.
Keywords
CD24, Hedgehog, hASC, adipose tissue, angiogenesis, UrSuppe, defined cell culture, serum- and xeno-free cell culture
Journal
Biologics
Volume
1
Number ( Month )
2
Pages (or article number)
129-153
Diffusion
License
CC BY
Visibility
Public
Status open access
Gold
